Probing off-target effect of LMNA siRNA and pro-siRNA in HeLa-d1EGFP cell line

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GSE44105

Homo sapiens

6 Downloadable Samples

Affymetrix Human Gene 1.0 ST Array (hugene10st)

Submitter Supplied Information

Description

We have develped a novel method of making siRNAs (named pro-siRNA for prokaryotic siRNA). To evaluate off-targeting of pro-siRNA, we compared mRNA expression profile of HeLa-d1EGFP cells transfected with 4 nM LMNA siRNAs and pro-siRNAs by microarray.

PubMed ID

23475073

Publication Title

Efficient and specific gene knockdown by small interfering RNAs produced in bacteria.

Total Samples

Submitter’s Institution

Boston Children's Hospital

Authors

Huang L, Jin J, Deighan P, Kiner E, McReynolds L, Lieberman J

Source Repository

Gene Expression Omnibus (GEO)

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