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Accession IconSRP003561

Probing the initiation and effector phases of the somatic piRNA pathway in Drosophila

Organism Icon Drosophila melanogaster
Sample Icon 8 Downloadable Samples
Technology Badge IconIllumina Genome Analyzer II

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Combining RNAi in cultured cells and analysis of mutant animals, we probed roles of known piRNA pathway components in the initiation and effector phases of transposon silencing. Overall design: total RNA and RNA associated with Piwi was isolated and size-fractionated by PAGE into 19-29nt. These were processed and sequenced on Illumina Genome Analyzer II.
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