Ribosomal footprinting of MDA_Ctrl and MDA_Arg overexpression cell lines

We used Illlumina Artseq Ribo Profile to perform ribosome footprinting on the above cell lines Overall design: We followed the manufacturer's protocol: Cells were treated with cycloheximide (0.1 mg/ml) to inhibit translation; total RNA(tt) and ribosome-protected RNA fragments (RPF) were isolated and library preped for sequencing simultaneously.

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Goodarzi H, Nguyen HCB, Zhang S, Dill BD, Molina H, Tavazoie SF