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Accession IconSRP155526

Reprogram-Seq: A platform for single-cell combinatorial reprogramming [I]

Organism Icon Mus musculus
Sample Icon 49 Downloadable Samples
Technology Badge IconNextSeq 500

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Description
Reprogram-Seq leverages organ-specific cell atlas data with single-cell perturbation and computational analysis to predict, evaluate, and optimize TF combinations that reprogram a cell type of interest. Overall design: Focusing on the cardiac system, we performed Reprogram-Seq on P0 mouse heart cells to generate a reference transcriptomic map. Based on the reference map, we selected TF candidates and tests 1000s of TF cocktails for direct lineage conversion by scRNA-Seq.
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49
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