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Accession IconSRP159243

RNA Sequencing Facilitates Quantitative Analysis of differentially expressed genes during human erythroipoiesis

Organism Icon Homo sapiens
Sample Icon 8 Downloadable Samples
Technology Badge IconIllumina Genome Analyzer IIx

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Description
Purpose: The goals of this study are to determine the differentially expreseed non-coding RNAs during human erythropoiesis Methods:gene expression profiling with cells on days 4,8,11,14 during erythroid differentiation, with two replicated using Illumina Genome Analyzer IIx. qRT–PCR validation was performed using TaqMan and SYBR Green assays. Conclusions: ncRNAs were differentially expressed during erythroid differentiation Overall design: mRNA profiles of day4,8,11,14 erythroid cells were generated by deep sequencing, in duplicate.
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