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Accession IconSRP164900

Effects of high fructose and high glucose on third instar larval fat body gene expression in Drosophila melanogaster

Organism Icon Drosophila melanogaster
Sample Icon 12 Downloadable Samples
Technology Badge IconIllumina Genome Analyzer IIx

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Description
We characterized monosaccharide-dependent gene expression in the Drosophila fat body using fructose and glucose. Control and high-sugar diets were compared and RNA-seq was used to identify potential target genes. Overall design: Drosophila were reared on control (0.3 M fructose or glucose) or high sugar (1.7 M fructose or glucose) diets until the wandering third instar stage. Fat bodies were isolated and RNA was extracted to determine the effects of each sugar at different concentrations on gene expression using Illumina RNA-seq.
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12
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